Rec-mediated recombinational activity of two adjacent Chi elements in bacteriophage lambda
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of the chi recombinational hot spot chi +D in bacteriophage lambda.
Chi sites in bacteriophage lambda stimulate recombination promoted by the RecBC pathway of Escherichia coli. Mutations which create these sites occur at four widely separated loci in lambda. We report here the nucleotide sequence surrounding the site of one of these loci, chi D, located near the S gene. The mutations creating the active Chi site, designated chi +D, are transversions from CG to ...
متن کاملSingle burst study of rec- and red-mediated recombination in bacteriophage lambda.
Single bursts from three-point crosses of bacteriophage lambda were analyzed for recombination of markers several thousand nucleotide pairs apart. Single recombination, mediated by the rec system of Escherichia coli, is usually reciprocal. Double recombinants are also significantly correlated in single bursts, although the correlation is weaker than for reciprocal singles. Reciprocity is not fo...
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Chi sites have previously been shown to stimulate homologous recombination by the Escherichia coli RecBC pathway. To test the activity of Chi in another organism, bacteriophage lambda crosses were carried out in Salmonella typhimurium strains bearing the E. coli lambda receptor protein. Chi is active in these crosses in S. typhimurium, but is less active than in the same crosses carried out in ...
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The recombination hotspot Chi, 5' G-C-T-G-G-T-G-G 3', stimulates the RecBCD recombination pathway of Escherichia coli. We have determined, with precision greater than previously reported, the distribution of Chi-stimulated exchanges around a Chi site in phage lambda. Crosses of lambda phages with single base-pair mutations surrounding a Chi site were conducted in and analyzed on mismatch correc...
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Although homologous recombination and DNA repair phenomena in bacteria were initially extensively studied without regard to any relationship between the two, it is now appreciated that DNA repair and homologous recombination are related through DNA replication. In Escherichia coli, two-strand DNA damage, generated mostly during replication on a template DNA containing one-strand damage, is repa...
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ژورنال
عنوان ژورنال: Genetical Research
سال: 1985
ISSN: 0016-6723,1469-5073
DOI: 10.1017/s0016672300021911